期刊
JOURNAL OF MOLECULAR CATALYSIS B-ENZYMATIC
卷 21, 期 1-2, 页码 47-49出版社
ELSEVIER SCIENCE BV
DOI: 10.1016/S1381-1177(02)00135-2
关键词
alcohol oxidase; methylotropic yeast; Pichia pastoris; immobilization; aldehyde
Alcohol oxidase (AO) from the methylotropic yeast Pichiapastoris was isolated and investigated. Wide substrate specificity is characteristic for this enzyme. Unbranched primary alcohols are effectively oxidized by AO to aldehydes, including propargyl alcohol, 2-chloroethanol, 2-cyanoethanol, leading to important synthetic intermediates. AO was immobilized by covalent linking to macroporous cellulose activated by glutaraldehyde, yield of immobilization 80%. Presence of two izoenzymes of AO was suggested from the pH activity dependence. (C) 2002 Elsevier Science B.V. All rights reserved.
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