期刊
BRAIN RESEARCH
卷 959, 期 1, 页码 58-67出版社
ELSEVIER SCIENCE BV
DOI: 10.1016/S0006-8993(02)03727-7
关键词
5-HT1A; fluorescence double-immunolabeling; confocal microscopy; colocalization; calcium binding protein
The 5-HT1A receptor is a well-characterized serotonin receptor playing a role in many central nervous functions and known to be involved in depression and other mental disorders. In situ hybridization, immunocytochemical, and binding studies have shown that the 5-HT1A receptor is widely distributed in the rat brain, with a particularly high density in the limbic system. The receptor's localization in the different neuronal subtypes, which may be of importance for understanding its role in neuronal circuitries, is, however, unknown. In this study we show by immunocytochemical double-labeling techniques, that the 5-HT1A receptor is present on both pyramidal and principal cells, and calbindin- and parvalbumin-containing neurons, which generally define two different subtypes of interneurons. Moreover, semiquantitative analysis showed that the receptor's distribution in the different neuronal types varies between brain areas. In cortex, hippocampus, hypothalamus, and amygdala the receptor was located on both principal cells and calbindin- and parvalbumin-containing neurons. In septum and thalamus, the receptor was mostly present on calbindin- and parvalbumin-containing cells. Especially in the medial septum and thalamic reticular nucleus, the receptor highly colocalized with parvalbumin-positive neurons. These results suggest a diverse function of the 5-HT1A receptor in modulating neuronal circuitry in different brain areas, that may depend on the type of neuron the receptor is predominantly located on. (C) 2002 Elsevier Science B.V. All rights reserved.
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