期刊
NEURON
卷 37, 期 1, 页码 85-97出版社
CELL PRESS
DOI: 10.1016/S0896-6273(02)01126-1
关键词
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资金
- NIGMS NIH HHS [1 F31 GM20596-03, GM35063, GM30376] Funding Source: Medline
Site-specific electrostatic measurements have been limited to soluble proteins purified for in vitro spectroscopic characterization or proteins of known structure; however, comparable measurements have not been made for functional membrane bound proteins. Here, using an electrochromic fluorophore, we describe a method to monitor localized electric field changes in a voltage-gated potassium channel. By coupling the novel probe Di-1-ANEPIA to cysteines in Shaker and tracking field-induced optical changes, in vivo electrostatic measurements were recorded with submillisecond resolution. This technique reports dynamic changes in the electric field during the gating process and elucidates the electric field profile within Shaker. The extension of this method to other membrane bound proteins, including transporters, will yield insight into the role of electrical forces on protein function.
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