期刊
EMBO JOURNAL
卷 22, 期 2, 页码 304-314出版社
OXFORD UNIV PRESS
DOI: 10.1093/emboj/cdg024
关键词
processivity; telomerase; telomerase RNA; telomeres; translocation
资金
- NIA NIH HHS [AG09383, R37 AG009383] Funding Source: Medline
Telomerase contains two essential components: an RNA molecule that templates telomeric repeat synthesis and a catalytic protein component. Human telomerase is processive, while the mouse enzyme has much lower processivity. We have identified nucleotide determinants in the telomerase RNA that are responsible for this difference in processivity. Mutations adjacent to the template region of human and mouse telomerase RNA significantly altered telomerase processivity both in vitro and in vivo. We also identified functionally important nucleotides in the pseudoknot domain of telomerase RNA that potentially mediate the incompatibility between human TERT and mouse telomerase RNA. These experiments identify essential residues of the telomerase RNA that regulate telomerase activity and processivity.
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