期刊
JOURNAL OF AOAC INTERNATIONAL
卷 98, 期 6, 页码 1529-1534出版社
AOAC INT
DOI: 10.5740/jaoacint.15-016
关键词
-
资金
- Iran National Science Foundation (INSF)
- Bakhtar Bioshimi Pharmaceutical Co., Kermanshah, Iran
A new, simple, and rapid chiral HPLC method was developed for enantioselective analysis of levetiracetam and its enantiomer [(R)-alpha-ethyl-2-oxo-pyrrolidine acetamide] in a pharmaceutical formulation and bulk material. Enantiomeric separation was achieved on a chiral-alpha(1)-acid glycoprotein (AGP) column (150 x 4.0 mm, 5 pm) using an isocratic mobile phase of phosphate buffer (pH = 7) at a flow rate of 0.7 mL/min. The UV detector was set at 210 nm. Calibration curves were linear in the range of 1-100 mu g/mL and 0.4-20 mu g/mL for levetiracetam and the (R)-enantiomer, respectively. LOD and LOQ for the (R)-enantiomer were 0.1 and 0.4 mu g/mL, respectively. The run time of analysis was less than 5.0 min.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据