期刊
CHEMISTRY-A EUROPEAN JOURNAL
卷 9, 期 2, 页码 475-483出版社
WILEY-V C H VERLAG GMBH
DOI: 10.1002/chem.200390050
关键词
avidin; cross-linker; iridium; luminescence; X-ray diffraction
We report the synthesis, characterization, photophysical, and electrochemical properties of a series of luminescent cyclometalated iridium(III) complexes containing two aldehyde functional groups [Ir(pba)(2)(N-N)](PF6) (Hpba = 4-(2-pyridyl)benzaldehyde; N-N=2,2'-bipyridine, bpy (1), 1,10-phenanthroline, phen (2), 3,4,7,8-tetramethyl-1,10-phenanthroline, 3,4,7,8-Me-4-phen (3), 4,7-diphenyl-1,10-phenanthroline, 4,7-Ph-2-phen (4)). The X-ray crystal structure of complex 1 has been investigated. Upon photoexcitation, complexes 1-4 exhibit intense and long-lived emission in fluid solutions at 298 K and in low-temperature glass. The luminescence is assigned to a triplet intra-ligand ((IL)-I-3) excited state associated with the pba(-) ligand, probably with mixing of some triplet metal-to-ligand charge-transfer ((MLCT)-M-3) (dpi(Ir)-->pi*(pba(-))) character. Since each of these complexes possesses two aldehyde groups, which can react with the primary amine groups of biomolecules to form stable secondary amines after reductive amination, we have investigated the possibility of these complexes as novel luminescent cross-linkers for biological substrates. L-Alanine has been labeled with complexes 1-4 to give the luminescent bioconjugates 1-(Ala)(2)-4-(Ala)(2). These conjugates show strong photoluminescence with long emission lifetimes under ambient conditions. On the basis of the emission energy trend, the excited state of these luminescent bioconjugates is likely to bear a high parentage of (MLCT)-M-3 (dpi(Ir)-->pi*(N-N)) character. In addition, the glycoprotein avidin (Av) has also been conjugated with complexes 1-4 to give the bioconjugates 1-Av-4-Av. Upon photoexcitation, these bioconjugates also display intense and long-lived (MLCT)-M-3 (dpi(Ir)-->pi*(N-N)) emission in aqueous buffer at 298 K. Furthermore, a heterogeneous competitive assay for biotin has been developed using 2-Av and biotinylated microspheres. We have shown that complexes 1-4 represent a new class of multicolor luminescent cross-linkers for biomolecular species.
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