期刊
JOURNAL OF ORGANIC CHEMISTRY
卷 68, 期 2, 页码 609-612出版社
AMER CHEMICAL SOC
DOI: 10.1021/jo026615r
关键词
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Click chemistry 1,3-dipolar cycloaddition between alkynyl 6-carboxyfluorescein (FAM) and azido-labeled single-stranded (ss) DNA was carried out under aqueous conditions to produce FAM-labeled ssDNA in quantitative yield. The FAM-labeled ssDNA was successfully used as a primer to produce DNA sequencing products with singlebase resolution in a capillary electrophoresis DNA sequencer with laser-induced fluorescence detection.
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