期刊
SCIENCE
卷 299, 期 5607, 页码 682-686出版社
AMER ASSOC ADVANCEMENT SCIENCE
DOI: 10.1126/science.1079700
关键词
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资金
- NCRR NIH HHS [P41-RR04224] Funding Source: Medline
Optical approaches for observing the dynamics of single molecules have required pico- to nanomolar concentrations of fluorophore in order to isolate individual molecules. However, many biologically relevant processes occur at micromolar ligand concentrations, necessitating a reduction in the conventional observation volume by three orders of magnitude. We show that arrays of zero-mode waveguides consisting of subwavelength holes in a metal film provide a simple and highly parallel means for studying single-molecule dynamics at micromolar concentrations with microsecond temporal resolution. We present observations of DNA polymerase activity as an example of the effectiveness of zero-mode waveguides for performing single-molecule experiments at high concentrations.
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