期刊
JOURNAL OF CHROMATOGRAPHY A
卷 988, 期 1, 页码 53-67出版社
ELSEVIER SCIENCE BV
DOI: 10.1016/S0021-9673(02)02050-2
关键词
retention behaviour; retention models; critical mobile phase composition; polydisperse macromolecules
This paper illustrates how conventional models of chromatographic behaviour can be used to predict the separation behaviour of polydisperse macromolecules. Using polystyrene and polymethylmethacrylate homo- and co-polymeric standards, the models were validated by comparing experimental retention behaviour with that predicted by the chromatographic model. The experimental retention time of each of the samples was entered into a spreadsheet application, which calculated the parameters that best described retention (for a given model). When a correlation between the relevant parameters and molecular mass was established, that correlation was used to predict the change in retention behaviour over the molecular-mass range. An expression introduced in a previous paper, to calculate the critical mobile-phase composition of a homopolymer was validated using polystyrene homopolymers. A second expression, which can predict the elution behaviour of copolymers, was also validated. This expression can predict the retention of a copolymer, based solely on the retention of the homopolymeric units that make up the copolymer. (C) 2002 Elsevier Science B.V. All rights reserved.
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