期刊
PLANT BREEDING
卷 122, 期 1, 页码 83-85出版社
BLACKWELL VERLAG GMBH
DOI: 10.1046/j.1439-0523.2003.00801.x
关键词
endopeptidase; eyespot resistance; Triticum aestivum; Aegilops ventricosa; genetic mapping; AFLP
Amplified fragment length polymorphism (AFLP) markers linked to the Aegilops ventricosa -derived chromosome segment in 'VPM1' on which the eyespot resistance gene, Pch1 , and the endopeptidase gene, Ep-D1b , occur were identified. One marker was isolated from the gel, cloned and sequenced. Sequence analysis revealed a microsatellite repeat motif. Sequence-specific primers were designed to amplify a product containing the repeat motif, and the microsatellite marker was tested for cosegregation with the Ep-D1b allele. Distinct alleles were produced by the Pch1 sources, normal wheat and wheat containing the Lr19 translocation. A recombination frequency of 0.02 was calculated between the microsatellite marker and Ep-D1 .
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