期刊
JOURNAL OF APPLIED PHYSIOLOGY
卷 94, 期 2, 页码 744-751出版社
AMER PHYSIOLOGICAL SOC
DOI: 10.1152/japplphysiol.00737.2002
关键词
sarcopenia; Fischer 344 x Brown Norway F1 hybrid rat; muscle blood flow; rat hindlimb; mitochondria
Although it is well established that maximal O-2 uptake ((V) over dot O-2 (max)) declines from adulthood to old age, the role played by alterations in skeletal muscle is unclear. Specifically, because during whole body exercise reductions in convective O-2 delivery to the working muscles from adulthood to old age compromise aerobic performance, this obscures the influence of alterations within the skeletal muscles. We sought to overcome this limitation by using an in situ pump-perfused hindlimb preparation to permit matching of muscle convective O-2 delivery in young adult (8 mo; muscle convective O-2 delivery = 569 +/- 42 mumol O-2.min(-1).100 g(-1)) and late middle-aged (28-30 mo; 539 +/- 62 mumol O-2.min(-1).100 g(-1)) Fischer 344 x Brown Norway F1 hybrid rats. The distal hindlimb muscles were electrically stimulated for 4 min (60 tetani/min), and (V) over dot O-2 (max) was determined. (V) over dot O-2 max normalized to the contracting muscle mass was 22% lower in the 28- to 30-mo-old (344 +/- 17 mumol O-2.min(-1).100 g(-1)) than the 8-mo-old (441 +/- 20 mumol O-2.min(-1).100 g(-1); P < 0.05) rats. The flux through the electron transport chain complexes I-III was 45% lower in homogenates prepared from the plantaris muscles of the older animals. Coincident with these alterations, the tension at (V) over dot O-2 max and lactate efflux were reduced in the 28- to 30-mo-old animals, whereas the percent decline in tension was greater in the 28- to 30-mo-old vs. 8-mo-old animals. Collectively, these results demonstrate that alterations within the skeletal muscles, such as a reduced mitochondrial oxidative capacity, contribute to the reduction in (V) over dot O-2 max with aging.
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