期刊
JOURNAL OF SURGICAL RESEARCH
卷 109, 期 2, 页码 92-100出版社
ACADEMIC PRESS INC ELSEVIER SCIENCE
DOI: 10.1016/S0022-4804(02)00036-7
关键词
burn injury; wound healing; insulin; polarization microscopy; muscle wasting
类别
资金
- NIGMS NIH HHS [R01 GM056442] Funding Source: Medline
Background. Insulin is proposed as a therapy for suppressing muscle wasting after burn trauma although the long-term effects of this therapy on wound healing are not yet known. The present study was designed to investigate the effect of systemically administered insulin therapy on burn wound healing. Materials and methods. Young rats weighing 80-150 g were subjected to 15-20% total body surface area burn injury on their shaved dorsum. The insulin dosage was increased over the first 3 days in each rat from 0.25 U (Day 1), 0.5 U (Day 2), and 1.0 U (Day 3) per 100 g body wt. The rats were euthanized at the fourth or fifteenth day postinjury. Skin sections were analyzed by histochemistry and quantitative polarization microscopy. Results. Histology showed a decreased number of inflammatory cells and increased vasodilation in the insulin-treated animals at Day 4 relative to untreated rats; at Day 15 there was increased reepithelialization. Quantitative analysis using polarization microscopy and picrosirius red staining showed an increased collagen deposition in wounds by Day 4 in insulin-treated rats relative to untreated burn controls. Conclusion. These results indicate that insulin induces accelerated wound healing associated with diminished inflammation and increased collagen deposition. (C) 2003 Elsevier Science (USA).
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