期刊
PLANT SCIENCE
卷 164, 期 2, 页码 253-257出版社
ELSEVIER IRELAND LTD
DOI: 10.1016/S0168-9452(02)00407-7
关键词
B-phycoerythrin (BPE); R-phycocyanin (RPC); BPE-RPC conjugate; phycobilisome (PBS); absorption spectrum; fluorescence emission spectrum
B-phycoerythrin (BPE) and R-phycocyanin (RPC) were purified from Porphyridium cruentum by Sephadex G-200 chromatography, then the BPE was attached covalently to the RPC by reacting their amino groups to form the artificially covalent BPE-RPC conjugate in which the excitation energy can transfer from the BPE to the RPC with low efficiency. Meanwhile, the intact phycobilisome (PBS) consisting of BPE, RPC, APC and L-CM was isolated and purified from Porphyridium cruentum, and the purified PBS was found to keep intact if the solution contains sucrose. Comparison of spectroscopic properties between the purified PBS and the BPE-RPC conjugate suggests that the BPE-RPC conjugate is much more stable than the purified PBS. The construction of BPE-RPC conjugate with low efficiency of the excitation energy transfer may be useful for preparing phycobiliprotein probes. (C) 2002 Elsevier Science Ireland Ltd. All rights reserved.
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