期刊
ASSAY AND DRUG DEVELOPMENT TECHNOLOGIES
卷 11, 期 2, 页码 101-107出版社
MARY ANN LIEBERT, INC
DOI: 10.1089/adt.2012.476
关键词
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资金
- Swiss Initiative in Systems Biology (SystemsX), through the Bridge-to-Industry project (BIP) grant entitled High-Content Screening by Digital Holographic imaging
- CTI program [12669.1 PFLS-LS]
We introduce a label-free technology based on digital holographic microscopy (DHM) with applicability for screening by imaging, and we demonstrate its capability for cytotoxicity assessment using mammalian living cells. For this first high content screening compatible application, we automatized a digital holographic microscope for image acquisition of cells using commercially available 96-well plates. Data generated through both label-free DHM imaging and fluorescence-based methods were in good agreement for cell viability identification and a Z'-factor close to 0.9 was determined, validating the robustness of DHM assay for phenotypic screening. Further, an excellent correlation was obtained between experimental cytotoxicity dose-response curves and known IC50 values for different toxic compounds. For comparable results, DHM has the major advantages of being label free and close to an order of magnitude faster than automated standard fluorescence microscopy.
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