期刊
FUNGAL GENETICS AND BIOLOGY
卷 38, 期 1, 页码 98-109出版社
ACADEMIC PRESS INC ELSEVIER SCIENCE
DOI: 10.1016/S1087-1845(02)00516-9
关键词
chitin; chitin synthesis; Aspergillus fumigatus; cell wall analysis
Previous studies (Aufauvre-Brown et al., 1997; Mellado et al., 1996a,b) have shown that only two genes of the Aspergillus fumigatus chitin synthase family, chsG and chsE, play a role in the morphogenesis of this fungal species. An A. fumigatus strain lacking both chsG (class III CHS) and chsE (class V CHS) genes was constructed by gene replacement of the chsE gene with a copy that has its conserved coding region interrupted by the hph resistance cassette in an A. fumigatus ChsG(-) genetic background. Unexpectedly the double disruption was not lethal. The double mutant AfchsG(-)IchsE(-) strain (i) has reduced chitin synthase activity with or without trypsin stimulation, (ii) has a reduced colony radial growth rate, (iii) produces highly branched hyphae, (iv) exhibits aberrant features, such as periodic swellings along the length of the hyphae and a block in conidiation that can be partially restored by an osmotic stabilizer (v) shows alterations in the shape and germination capacity of the conidia, and (vi) has a cell wall that contains half the chitin of the parental strain and is, unexpectedly, highly enriched in alpha-(1-3) glucan. (C) 2002 Elsevier Science (USA). All rights reserved.
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