期刊
JOURNAL OF COMPARATIVE PATHOLOGY
卷 128, 期 2-3, 页码 182-187出版社
ELSEVIER SCI LTD
DOI: 10.1053/jcpa.2002.0624
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资金
- NIAID NIH HHS [R01 AI3001] Funding Source: Medline
Specific identification of ehrlichiae in the tissues and determination of their distribution is difficult. In this study, an in-situ hybridization method was developed to detect ehrlichial 16S rRNA in tissue specimens from mice experimentally infected with the HF strain. This strain is closely related to Ehrlichia chaffeensis, the agent of human monocytic ehrlichlosis. HF strain-specific 16S rRNA was detected in endothelial cells and monocyte-macrophages in the liver, lungs, bone marrow, spleen, lymph nodes., and large and small intestinal tissues. The results suggest that the in-situ hybridization method with a digoxigenin-labelled RNA probe specific to ehrlichial 16S rRNA will lie useful for post-mortem diagnosis and for the histopathological investigation of ehrlichial infection. (C) 2003 Elsevier Science Ltd. All rights reserved.
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