期刊
GENES & DEVELOPMENT
卷 17, 期 3, 页码 394-409出版社
COLD SPRING HARBOR LAB PRESS
DOI: 10.1101/gad.1044903
关键词
limb; Wnt/beta-catenin signaling; apical ectodermal ridge; mouse
资金
- NICHD NIH HHS [F32 HD08479, HD03249, F32 HD008479] Funding Source: Medline
- NIDDK NIH HHS [P01 DK056246, DK56246] Funding Source: Medline
- NINDS NIH HHS [R01 NS033642, R37 NS033642, NS 33642] Funding Source: Medline
The formation of the apical ectodermal ridge (AER) is critical for the distal outgrowth and patterning of the vertebrate limb. Recent work in the chick has demonstrated that interplay between the Wnt and Fgf signaling pathways is essential in the limb mesenchyme and ectoderm in the establishment and perhaps the maintenance of the AER. In the mouse, whereas a role for Fgfs for AER establishment and function has been clearly demonstrated, the role of Wnt/beta-catenin signaling, although known to be important, is obscure. In this study, we demonstrate that Wnt3, which is expressed ubiquitously throughout the limb ectoderm, is essential for normal limb development and plays a critical role in the establishment of the AER. We also show that the conditional removal of beta-catenin in the ventral ectodermal cells is sufficient to elicit the mutant limb phenotype. In addition, removing beta-catenin after the induction of the ridge results in the disappearance of the AER, demonstrating the requirement for continued beta-catenin signaling for the maintenance of this structure. Finally, we demonstrate that Wnt/beta-catenin signaling lies upstream of the Bmp signaling pathway in establishment of the AER and regulation of the dorsoventral polarity of the limb.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据