期刊
FASEB JOURNAL
卷 17, 期 2, 页码 136-143出版社
FEDERATION AMER SOC EXP BIOL
DOI: 10.1096/fj.01-0847com
关键词
Fas defects; EGFR; antibody library
资金
- NCI NIH HHS [CA-80312] Funding Source: Medline
- NHLBI NIH HHS [HL-44126] Funding Source: Medline
- NIAID NIH HHS [AI-31268] Funding Source: Medline
- NIAMS NIH HHS [P50AR44888] Funding Source: Medline
Autoantibodies to the recombinant extracellular domain of epidermal growth factor receptor (exEGFR) were detected by ELISA in the serum of Fas-defective old MRL/MpJ/lpr and C3H/HeJ/gld mice, but not young mice from these strains, or nonautoimmune young and old BALB/c, MRL/MpJ/++, and C3H/HeJ/MMTV mice. Compared with control human subjects without autoimmune disease, the frequency of exEGFR-binding autoantibodies was increased in scleroderma (systemic sclerosis) patients and to a lesser extent in lupus patients. Phage autoantibodies (Fv fragments) isolated from a lupus library by selection on a linear epitope of EGFR (residues 294310) displayed the ability to bind exEGFR. Treatment of EGFR-expressing A431 cells with autoantibodies purified by affinity chromatography on immobilized exEGFR resulted in specific staining of the cells. Short-lived but strong inhibition of cellular DNA synthesis was observed in the presence of the autoantibodies. We concluded that autoantibody responses to EGFR hold the potential of fulfilling a pathogenic role in autoimmune disease.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据