期刊
FUNGAL GENETICS AND BIOLOGY
卷 38, 期 1, 页码 75-84出版社
ACADEMIC PRESS INC ELSEVIER SCIENCE
DOI: 10.1016/S1087-1845(02)00512-1
关键词
bacterial artificial chromosome; chromosome walking; late blight; mating type; oomycete; synteny; translocation
Genetic and physical irregularities associated with the mating type locus of the oomycete, Phytophthora infestans, were revealed by analyzing a contig spanning the locus that was constructed using a bacterial artificial chromosome library. Contigs from both homologs of an A1 strain A/a genotype at mating type locus) had chromosome-specific differences, flanked by regions of similarity. Such heteromorphism was detected within multiple isolates. The mating type locus was narrowed to a 60-70 kb interval by genetic mapping of candidate genes, identified using a cDNA library. During these analyses, an unusual isolate of P. infestans was identified in which the mating type determinant had apparently translocated from its location in typical strains. Comparative mapping of the cDNAs between P. infestans and P. parasitica revealed partial synteny between the species however; substantial rearrangements existed and no cDNA was tightly linked to mating type in P. parasitica. These findings add to previous observations of unusual genetic behavior involving mating type in Phytophthora. (C) 2002 Elsevier Science (USA). All rights reserved.
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