期刊
ASIAN PACIFIC JOURNAL OF CANCER PREVENTION
卷 15, 期 22, 页码 9853-9857出版社
ASIAN PACIFIC ORGANIZATION CANCER PREVENTION
DOI: 10.7314/APJCP.2014.15.22.9853
关键词
Ribosomal protein L24; drug resistance mechanism; liver cancer; HepG2 cells
类别
资金
- National Natural Science Foundation of China [81271876]
- Natural Science Foundation of Shandong Province, China [ZR2011HL004, ZR2013CM031]
- higher education institution science and technology development project of Shandong Province [J13LF12]
Background: The morbidity and mortality rate of liver cancer continues to rise in China and advanced cases respond poorly to chemotherapy. Ribosomal protein L24 has been reported to be a potential therapeutic target whose depletion or acetylation inhibits polysome assembly and cell growth of cancer. Materials and Methods: Total RNA of cultured amycin-resistant and susceptible HepG2 cells was isolated, and real time quantitative RT-PCR were used to indicate differences between amycin-resistant and susceptible strains of HepG2 cells. Viability assays were used to determine amycin resistance in RPL24 transfected and control vector and null-transfected HepG2 cell lines. Results: The ribosomal protein L24 transcription level was 7.7 times higher in the drug-resistant HepG2 cells as compared to susceptible cells on quantitative RT-PCR analysis. This was associated with enhanced drug resistance as determined by methyl tritiated thymidine (3H-TdR) incorporation. Conclusions: The ribosomal protein L24 gene may have effects on drug resistance mechanisms in hepatocellular carcinoma HepG2 cells.
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