期刊
GLYCOBIOLOGY
卷 13, 期 2, 页码 129-137出版社
OXFORD UNIV PRESS INC
DOI: 10.1093/glycob/cwg005
关键词
electrospray mass spectrometry; liver fluke glycolipids; oligosaccharide structural analysis; parasitic trematode
The acidic (glyco)lipids of the parasitic liver fluke Fasciola hepatica exhibited two different phosphate-containing species, designated AL-I and AL-II, which were analyzed by MALDI-TOF MS, ESI MS, NMR, methylation analysis, and combined GC-MS in conjunction with HF treatment. AL-I was structurally determined as 1-O-hexadecyl-snglycerol-3-phosphoinositol, an ether bond variant of lysophosphatidylinositol. The structure of AL-II was shown to be GlcNAcalpha1-HPO3-6Gal(1-1)ceramide. Ceramide analysis revealed as major components 2-hydroxyoctadecanoic acid [18:0(2-OH)] together with C-18- and C-20-phytosphingosines. AL-II was apparently highly antigenic and strongly recognized by both animal- and human-F. hepatica infection sera. Furthermore, inhibition ELISAs revealed that the unusual antigenic determinant GlcNAcalphal-HPO3- phosphate might have a potential in the serodiagnosis of F. hepatica infections.
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