Cytotoxic and antiplatelet aggregation principles of Ruta graveolens

Nineteen compounds have been isolated from the methanol extract of the root and aerial parts of Ruta graveolens. The structural elucidation of these isolated compounds were determined by the spectroscopic methods and/or comparison of the physical data with literature values. Their antiplatelet aggregation and cytotoxic activities were examined to find potent antiplatelet aggregation and cytotoxic compounds from natural resources. Among them, dictamine (5), skimmianine (7), psoralen (8), chalepensin (12), clausindin (13), and graveolinine (16) showed significant inhibition of platelet aggregation, induced by arachidonic acid and collagen. Arborinine (2), dictamine (5), isopimpinellin (11), clausindin (13), and graveoline (17) exhibited cytotoxic activity against KB, Hela, DLD, NCl and Hepa tumor cell lines.