期刊
DNA REPAIR
卷 2, 期 2, 页码 159-173出版社
ELSEVIER SCIENCE BV
DOI: 10.1016/S1568-7864(02)00193-3
关键词
oxidation damage; 8-oxodGTP; 8-oxoguanine; mutators; spontaneous mutations
We have investigated in detail the interactions between the Escherichia coli mutT, mutM, and mutY error-prevention systems. Jointly, these systems protect the cell against the effects of the oxidative stress product, 8-oxoguanine (8-oxoG), a base analog with ambiguous base-pairing properties, pairing with either A or C during DNA synthesis. mutT mutator strains display a specific increase in A.T --> C.G transversions, while mutM and mutY mutator strains show specific G.C --> T.A increases. To study in more detail the in vivo processing of the various mutational intermediates leading to A.T --> C.G and G.C --> T.A transversions, we analyzed defined A.T --> C.G and G.C --> T.A events in strains containing all possible combinations of these mutator alleles. We report three major findings. First, we do not find evidence that the mutT allele significantly increases G.C --> T.A transversions in either mut(+), mutM, mutY or mutMmutY backgrounds. We interpret this result to indicate that incorporation of 8-oxodGTP opposite template C may not be frequent relative to incorporation opposite template A. Second, we show that mutT-induced A.T --> C.G transversions are significantly reduced in strains carrying mutY and mutMmutY deficiencies suggesting that 8-oxoG, when present in DNA, preferentially mispairs with dATP. Third, the mutY and mutMmutY deficiencies also decrease A.T --> C.G transversions in the muT(+) background, suggesting that, even in the presence of functional MutT protein, A.T --> C.G transversions may still result from 8-oxodGTP misincorporation. (C) 2002 Elsevier Science B.V. All rights reserved.
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