期刊
CARBOHYDRATE RESEARCH
卷 338, 期 4, 页码 341-352出版社
ELSEVIER SCI LTD
DOI: 10.1016/S0008-6215(02)00461-5
关键词
cell wall; oligosaccharide; NMR; mass spectrometry; arabinose flexibility
资金
- NCRR NIH HHS [RR05351] Funding Source: Medline
- NIGMS NIH HHS [GM33225] Funding Source: Medline
A 2-O-methylfucosyl-containing heptasaccharide was released from red wine rhamnogalacturonan II (RG-II) by acid hydrolysis of the glycosidic linkage of the aceryl acid residue (AceA) and purified to homogeneity by size-exclusion and high-performance anion-exchange chromatographies. The primary structure of the heptasaccharide was determined by glycosyl-residue and glycosyl-linkage composition analyses, ESIMS, and by H-1 and C-13 NMR spectroscopy. The NMR data indicated that the pyranose ring of the 2,3-linked L-arabinosyl residue is conformationally flexible. The L-Arap residue was confirmed to be alpha-linked by NMR analysis of a tetraglycosyl-glycerol fragment, [alpha-L-Arap-(1 --> 4)-beta-D-Galp-(1 --> 2)-alpha-L-AcefA-(1 --> 3)-beta-L-Rhap-(1 --> 3)-Gro], generated by Smith degradation of RG-II. Our data together with the results of a previous study,(1) establish that the 2-O-Me Fuc-containing nonasaccharide side chain of wine RG-II has the structure (Api drop apiose): [GRAPHICS] Data are presented to show that in Arabidopsis RG-II the predominant 2-O-MeFuc-containing side chain is a mono-O-acetylated heptasaccharide that lacks the non-reducing terminal beta-L-Araf and the alpha-L-Rhap residue attached to the O-3 of Arap, both of which are present on the wine nonasaccharide. (C) 2003 Elsevier Science Ltd. All rights reserved.
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