期刊
NEUROREPORT
卷 14, 期 2, 页码 257-260出版社
LIPPINCOTT WILLIAMS & WILKINS
DOI: 10.1097/00001756-200302100-00020
关键词
Alzheimer's disease; CHO-K1; COS-7; glycogen synthase kinase-3 beta; SH-SY5Y; tau phosphorylation
资金
- Medical Research Council [G0000749] Funding Source: Medline
To study further the role of glycogen synthase kinase-3beta on tau phosphorylation, glycogen synthase kinase-3beta and tau expression vectors were co-transfected into CHO-KI, COS-7 and SH-SY5Y cell. Tau phosphorylation was assessed by phosphorylation-dependent antibodies AT-8, AT-180, AT-270 and PHF-I. The AT-270 and AT-8 epitopes were consistently phosphorylated by glycogen synthase kinase-3beta in the three cell lines. Phosphorylation on AT- 180 epitope was significant in CHO-KI and SH-SY5Y cells while PHF-I epitope was hyper-phosphorylated only in SH-SY5Y cells. We also found that lithium induces phosphorylation of the serine 9 residue of glycogen synthase kinase-3beta together with inhibition of tau phosphorylation on PHF-1 epitope in all the three cell lines. This suggests a novel mechanism whereby lithium-mediated inhibition of GSK-3beta activity influences tau phosphorylation.
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