期刊
EMBO JOURNAL
卷 22, 期 4, 页码 954-963出版社
OXFORD UNIV PRESS
DOI: 10.1093/emboj/cdg091
关键词
DNA binding; DNA replication; initiator; specificity factor
资金
- NCI NIH HHS [CA13106, P01 CA013106] Funding Source: Medline
Initiator proteins are critical components of the DNA replication machinery and mark the site of initiation. This activity probably requires highly selective DNA binding; however, many initiators display modest specificity in vitro. We demonstrate that low specificity of the papillomavirus E1 initiator results from the presence of a non-specific DNA-binding activity, involved in melting, which masks the specificity intrinsic to the E1 DNA-binding domain. The viral factor E2 restores specificity through a physical interaction with E1 that suppresses non-specific binding. We propose that this arrangement, where one DNA-binding activity tethers the initiator to ori while another alters DNA structure, is a characteristic of other viral and cellular initiator proteins. This arrangement would provide an explanation for the low selectivity observed for DNA binding by initiator proteins.
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