期刊
ASIAN PACIFIC JOURNAL OF CANCER PREVENTION
卷 14, 期 11, 页码 6945-6948出版社
ASIAN PACIFIC ORGANIZATION CANCER PREVENTION
DOI: 10.7314/APJCP.2013.14.11.6945
关键词
Lung cancer; hTERT; diosgenin; telomerase; qRT-PCR; MTT assay
类别
资金
- Drug Applied Research Center, Tabriz University of Medical Sciences, Tabriz, Iran
Background: Diosgenin, a steroidal saponin from a therapeutic herb, fenugreek (Trigonellafoenum-graceum L.), has been recognized to have anticancer properties. Telomerase activity is not detected in typical healthy cells, while in cancer cell telomerase expression is reactivated, therefore providing a promising cancer therapeutic target. Materials and Methods: We studied the inhibitory effect of diosgenin on human telomerase reverse transcriptase gene (hTERT) expression which is critical for telomerase activity. MTT- assays and qRT-PCR analysis were conducted to assess cytotoxicity and hTERT gene expression inhibition effects, respectively. Results: MTT results showed that IC50 values for 24, 48 and 72h after treatment were 47, 44 and 43 mu M, respectively. Culturing cells with diosgenin treatment caused down-regulation of hTERT expression. Discussion: These results show that diosgenin inhibits telomerase activity by down-regulation of hTERT gene expression in the A549 lung cancer cell line
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