期刊
JOURNAL OF THE AMERICAN CHEMICAL SOCIETY
卷 125, 期 7, 页码 1778-1787出版社
AMER CHEMICAL SOC
DOI: 10.1021/ja0287377
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资金
- NIGMS NIH HHS [GM65519] Funding Source: Medline
A second-generation fluorescent sensor for Zn2+ from the Zinpyr family, ZP4, has been synthesized and characterized. ZP4 (Zinpyr-4, 9-(o-carboxyphenyl)-2-chloro-5-[2-{bis(2-pyridylmethyl)-aminomethyl}-N-methylaniline]-6-hydroxy-3-xanthanone) is prepared via a convergent synthetic strategy developed from previous studies with these compounds. ZP4, like its predecessors, has excitation and emission wavelengths in the visible range (similar to500 nm), a dissociation constant (K-d) for Zn2+ of less than 1 nM and a high quantum yields (Phi = similar to0.4), making it well suited for biological applications. A 5-fold fluorescent enhancement is observed under simulated physiological conditions corresponding to the binding of the Zn2+ cation to the sensor, which inhibits a photoinduced electron transfer (PET) quenching pathway. The metal-binding stereochemistry of ZP4 was evaluated through the synthesis and X-ray structural characterization of [M(BPAMP)(H2O)(n)](+) Complexes, where BPAMP is [2-{bis(2-pyridylmethyl)aminomethyl}-N-methylaniline]-phenol and M = Mn2+, Zn2+ (n = 1) or Cu2+ (n = 0).
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