期刊
JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY
卷 51, 期 5, 页码 1162-1168出版社
AMER CHEMICAL SOC
DOI: 10.1021/jf025997z
关键词
LC/MS/MS; strigol; orobanchol; sorgolactone; parasitic weeds; germination stimulants; Striga spp.; Orobanche spp.
A simple and rapid analytical method for strigolactones, germination stimulants for the root parasitic weeds witchweed (Striga spp.) and broomrape (Orobanche spp.), has been developed using highperformance liquid chromatography connected to tandem mass spectrometry (LC/MS/MS). The natural strigolactones (strigol, sorgolactone, orobanchol, and alectrol) were clearly separated and identified by LC/MS/MS. As low as 0.1 pg/muL of strigol and 0.5 pg/muL of sorgolactone could be quantified, whereas 1 pg/muL was needed for the quantification of orobanchol (S/N > 10). Using this method, it was found that red clover produces orobanchol and alectrol but not strigol. The roots of red clover seedlings were found to produce 13, 70, 58, and 65 pg of orobanchol/plant 1, 2, 3, and 4 weeks after germination, respectively.
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