期刊
JOURNAL OF TOXICOLOGY AND ENVIRONMENTAL HEALTH-PART A-CURRENT ISSUES
卷 66, 期 4, 页码 365-378出版社
TAYLOR & FRANCIS INC
DOI: 10.1080/15287390306362
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Since alveolar macrophages play an important role in the clearance of inhaled dust from airways, these cells have been used as a target for various toxic chemicals. Alveolar macrophages; obtained from bronchoalveolar lavage of Syrian golden hamsters were concurrently exposed in vitro to Fe3O4, as an indicator for magnetometry, and various concentrations of cadmium oxide (CdO) in this study. A rapid decrease of the remnant magnetic field, called relaxation, was observed after the cessation of an external magnetic field stimulus in macrophages concurrently exposed to phosphate-buffered saline or CdO at 0.1 mug/ml, while relaxation was delayed in those concurrently exposed to 1, 25, or 50 mug/ml CdO. Therefore, the concentration of CdO affecting relaxation in vitro was estimated at between 0.1 and 1 mug/ml. Release of LDH activity from CdO-exposed macrophages into the medium significantly increased at levels of 25 and 50 mug/ml CdO. Apoptosis was not detected in macrophages exposed to CdO by the DNA ladder detection method or morphological observations. Electron-microscopic examination revealed severe membrane damage and vacuolar changes in macrophages exposed to CdO. Since delayed relaxation is thought to occur by (1) disrupted cytoskeleton-driven random rotation of phagosomes containing iron oxide particles, (2) significant lactate dehydrogenase (LDH) activity release, and (3) detachment of cell membranes, CdO is considered to affect macrophage functions.
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