期刊
JOURNAL OF NEUROPHYSIOLOGY
卷 89, 期 3, 页码 1214-1222出版社
AMER PHYSIOLOGICAL SOC
DOI: 10.1152/jn.00516.2002
关键词
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In the mechanically dissociated rat hippocampal CA1 neurons with native presynaptic nerve endings, namely synaptic bouton preparation, the purinergic modulation of spontaneous GABAergic miniature inhibitory postsynaptic currents (mIPSCs) was investigated using whole-cell recording mode under the voltage-clamp conditions. In immature neurons, adenosine (10 muM) reversibly decreased GABAergic mIPSC frequency without affecting the mean current amplitude. The inhibitory effect of adenosine transmission was completely blocked by 8-cyclopentyl-1,3-dipropylxanthine (DPCPX, 100 nM), a selective A, receptor antagonist, and was mimicked by N-6-cyclopentyladeno sine (CPA, 1 muM), a selective A(1) receptor agonist. However, CPA had no effect on GABAergic mIPSC frequency in postnatal 30 day neurons. N-ethylmaleimide (10 muM), a guanosine 5'-triphosphate binding protein uncoupler, and Ca2+-free external solution removed the CPA-induced inhibition of mIPSC frequency. K+ channel blockers, 4-aminopyridine (100 muM) and Ba2+ (1 mM), had no effect on the inhibitory effect of CPA on GABAergic mIPSC frequency. Stimulation of adenylyl cyclase with forskolin (10 muM) prevented the CPA action on GABAergic mIPSC frequency. Rp-cAMPS (100 muM), a selective PKA inhibitor, also blocked the CPA action. It was concluded that the activation of presynaptic A(1) receptors modulates the probability of spontaneous GABA release via cAMP- and protein kinase A dependent pathway. This A(1) receptor-mediated modulation of GABAergic transmission may play an important role in the regulation of excitability of immature hippocampal CA1 neurons.
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