期刊
JOURNAL OF IMMUNOLOGICAL METHODS
卷 274, 期 1-2, 页码 177-184出版社
ELSEVIER
DOI: 10.1016/S0022-1759(02)00513-6
关键词
interieukin-7; IL-7; thymocyte; thymus; T cell development; apoptosis
In order to study the response of T cells to IL-7, we aimed to generate an IL-7-dependent thymocyte line. CD4(-)CD8(-)thymocytes from a p53(-)/(-) mouse were continuously propagated in interleukin-7 (IL-7), and after 2 months there developed an immortal line termed D1. The D1 line has retained a stable dependency on IL-7. Withdrawal of IL-7 from D1 cells induced arrest in G1 phase of the cell cycle, followed by apoptosis. In addition to IL-7, several other cyrokines that employ gamma(e) as part of their receptor were also capable of stimulating D I cell survival and proliferation. Gene induction by IL-7 was analyzed in D1 cells using RNase protection and array analysis and revealed a number of transcripts potentially involved in cell cycle, apoptosis and signaling. (C) 2002 Elsevier Science B.V. All rights reserved.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据