期刊
EUROPEAN JOURNAL OF PHARMACEUTICAL SCIENCES
卷 18, 期 3-4, 页码 241-249出版社
ELSEVIER SCIENCE BV
DOI: 10.1016/S0928-0987(03)00017-4
关键词
photodynamic therapy; meso-tetra(4-hydroxyphenyl)porphyrin; nanoparticles; poly(D,L-lactide-co-glycolide); poly(D,L-lactide); EMT-6-mammary tumour cells
A photosensitizer, meso-tetra(hydroxyphenyl)porphyrin (p-THPP) was incorporated into sterile submicronic nanoparticles of poly(D,L-lactide-co-glycolide) (50:50 and 75:25 PLGA) and poly(D,L-lactide) (PLA). With all polymers used, sub-130 nm p-THPP-loaded nanoparticles with similar drug loadings and entrapment efficiencies were produced using the emulsification-diffusion technique. The photodynamic activity (photocytotoxicity) of these nanoparticles was evaluated on EMT-6 mammary tumour cells in comparison with the free drug. The influence of drug concentration (3-10 mug/ml), incubation time (5-60 min) and light dose (6-9 J/cm(2)) on p-THPP photocytotoxic efficiency was investigated. With all p-THPP formulations tested, cell viability decreased with increasing values of these parameters. The beneficial effect of nanoencapsulation compared to free drug was highlighted at drug concentrations up to 6 mug/ml and short incubation times (15-30 min). The most important photocytotoxicity was observed with 50:50 PLGA nanoparticles allowing low drug doses and short drug administration-irradiation intervals for local photodynamic therapy. (C) 2003 Elsevier Science B.V. All rights reserved.
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