期刊
JOURNAL OF IMMUNOLOGICAL METHODS
卷 274, 期 1-2, 页码 63-75出版社
ELSEVIER SCIENCE BV
DOI: 10.1016/S0022-1759(02)00501-X
关键词
hapten; 11-deoxycortisol; monoclonal antibody; anti-idiotype antibody; noncompetitive immunoassay; ELISA
Anti-idiotype antibodies recognizing the variable regions of a particular anti-hapten antibody are valuable tools, which can be used in sensitive hapten immunoassays based on a noncompetitive formal. Here, we describe the production and characterization of monoclonal anti-idiotype antibodies against idiotopes on the variable regions of an antibody showing high affinity and specificity to 11-deoxycortisol (11-DC). 11-DC is the biosynthetic precursor of cortisol and a diagnostic index for the assessment of pituitary-adrenal function. BALB/c or A/J mice were repeatedly immunized with the anti-11-DC antibody conjugated with keyhole limpet hemocyanin and their spleen cells were then fused with P3/NS1/1-Ag4-1 myeloma cells. Seven kinds of antiidiotype antibodies were generated, one of which was a p-type antibody recognizing the paratope and others which were U-type antibodies recognizing the framework region. A noncompetitive ELISA based on idiotype-anti-idiotype reactions was established using one of these alpha-type antibodies in combination with the beta-type antibody and with the anti-11-DC antibody. This noncompetitive assay system provided improved sensitivity (detection limit: 1.0 pg = 2.9 fmol), which is approximately 10 times higher than the corresponding competitive enzyme immunoassay, and offered a practical specificity for clinical use. Appropriate serum 11-DC levels were obtained for normal subjects [0.16 +/- 0.09 (S.D.) mug/l (n = 6), ranging from 0.086 to 0.316 mug/l] using the present assay system. (C) 2002 Elsevier Science B.V. All rights reserved.
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