期刊
DEVELOPMENTAL DYNAMICS
卷 226, 期 3, 页码 470-477出版社
WILEY-LISS
DOI: 10.1002/dvdy.10254
关键词
morpholinos; chick embryos; delivery methods
资金
- NIGMS NIH HHS [GM53258] Funding Source: Medline
The use of antisense morpholino oligos to inhibit the translation of a target transcript has been applied recently to studies of the chicken embryo. In contrast to other developmental systems such as in frog, sea urchin, and zebrafish that permit the direct microinjection of morpholinos into a blastomere, square pulse electroporation is used to introduce fluorescently tagged morpholinos into specific populations of chick embryo cells in ovo. This article reviews the methods that have proven successful, the types of controls that are necessary when performing knockdowns of gene expression in the chick embryo, and discusses the limitations of the current technique, as well as directions for further research. (C) 2003 Wiley-Liss, Inc.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据