期刊
JOURNAL OF FOOD SCIENCE
卷 68, 期 2, 页码 487-490出版社
WILEY
DOI: 10.1111/j.1365-2621.2003.tb05699.x
关键词
DPPH; oxygen; light; free radicals; antioxidant activity measuring method
The absorbance of 2,2-diphenyl-1-picrylhydrazyl (DPPH) at 517 nm in methanol and acetone decreased by 20 and 35% for 120 min at 25degreesC under light, respectively; in the dark it did not change significantly for 150 min. Decomposition of DPPH under 21% oxygen after 90 min under light was significantly higher than that under 1% oxygen. Absorbance of DPPH in pH 4 buffer solution in methanol, and in pH 10 buffer solution in acetone, decreased by 55. and 80%, respectively, under light for 120 min. The evaluation of antioxidant activity by the changes of DPPH-absorbance, should be carefully interpreted since the absorbance of DPPH at 517 nm is decreased by light, oxygen, pH, and type of solvent in addition to the antioxidant.
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