期刊
ARCHIVES OF BIOCHEMISTRY AND BIOPHYSICS
卷 411, 期 1, 页码 129-135出版社
ELSEVIER SCIENCE INC
DOI: 10.1016/S0003-9861(02)00691-4
关键词
Helicobacter pylori; respiration; nitric oxide; cytochrome c oxidase; oxidative stress; superoxide; mitochondria; gastric ulcer
The aim of the present work is to elucidate the mechanism by which the respiration of Helicobacter pylori but not of Escherichia coli shows a strong resistance to nitric oxide (NO). Nitric oxide strongly but reversibly inhibited the oxygen consumption by sonicated membranes from H. pylori and Triton X-100-treated cells. Although the sensitivity of the H. pylori respiration to cyanide was low, it also increased after the treatment with Triton X-100. Kinetic analyses revealed that NO was rapidly degraded by E coli and the Triton X-100-treated H. pylori, but not by the intact H. pylori. Thus, the low sensitivity to NO might reflect the low affinity of the cytochrome c oxidase for this radical within the membrane/lipid bilayers of H. pylori. Such properties of the oxidase in H. pylori membranes may, at least in part, underlie the mechanism by which this bacterium thrives in NO-enriched gastric juice. (C) 2002 Elsevier Science (USA). All rights reserved.
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