期刊
WORLD JOURNAL OF MICROBIOLOGY & BIOTECHNOLOGY
卷 19, 期 2, 页码 117-121出版社
SPRINGER
DOI: 10.1023/A:1023284723636
关键词
bioaccumulation; biosorption; copper; metal-microbe interactions; metal resistance; Pseudomonas cepacia; Pseudomonas spp.
Biosorption of copper by Pseudomonas cepacia was found to be dependent on added copper concentration. Copper uptake by the cells was rapid over the range of copper concentrations tested and complete within the first 10 min of incubation time. The effect of pH on copper uptake by P. cepacia was determined using overlapping buffers over the pH range 3-8, and copper biosorption from a 10 mM copper solution was greatest at pH 7. Copper uptake (measured by analysis of cell digests) was unaffected by cyanide and azide (up to 30 mM) and by incubation of cells with a 10 mM copper solution at 4degreesC. Evidence from these results suggested that copper uptake by P. cepacia cells involves surface binding and not intracellular accumulation by active transport. Biosorption of copper by various Pseudomonas isolates from metal-contaminated environments agreed well with copper biosorption by Pseudomonas strains from the National Collection of Type Cultures (NCTC).
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