期刊
ASIAN JOURNAL OF CHEMISTRY
卷 25, 期 12, 页码 6807-6810出版社
ASIAN JOURNAL OF CHEMISTRY
DOI: 10.14233/ajchem.2013.14711
关键词
Corn; Fumonisins; Matrix solid-phase dispersion; High performance liquid chromatography; Photo-diode array detector
资金
- National Natural Science Foundation of China [30800769, 31071546]
A simple, rapid and reproducible analytical method for the determination of fumonisin B-1 (FB1) and fumonisin B-2 (FB2) in corn samples by matrix solid-phase dispersion procedure coupled to high performance liquid chromatography (HPLC) with photo-diode array detector (DAD). Several dispersants, eluents and ratios were tested during the optimization of the process in order to obtain the best results. Finally, samples were blended with C-18 and the mycotoxins were extracted with 10 mL of 10 mM formic acid in methanol. Analyses were performed by HPLC-DAD. The mobile phase was a mixture of a 77 % methanol and 23 % 0.1 M NaH2PO4 (pH 3.2) solution at 1 mL min(-1). The wavelength of detection was 335 nm. The recoveries of the extraction process ranged from 71.0 to 119.7 % with relative standard deviation lower than 15.7 % in all cases, when samples were fortified at three different concentration levels. Limits of detection and quantification were 0.05, 1 and 0.2, 2.0 mu g g(-1), respectively. Application of the method to the analysis of 13 samples including, corn kernel, flour, corn starch, popcorn, purchased in local supermarkets in Guangzhou city, China, revealed FB1 and FB2 levels.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据