期刊
JOURNAL OF ENDOCRINOLOGY
卷 176, 期 3, 页码 405-414出版社
BIOSCIENTIFICA LTD
DOI: 10.1677/joe.0.1760405
关键词
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Proopiomelanocortin (POMC) cDNA was cloned front sea bass (Dicentrarchus labrax) pituitary gland. A 743 nucleotide sequence was obtained coding for the following sequences flanked by sets of proteolytic cleavage sites: ACTH (Ser(88)-Met(127)), alpha-MSH (Ser(88)-Gly(102)), CLIP (Pro(106)-Met(127)), beta-LPH (Glu(131)-Gln(208)), gamma-LPH (Glu(131)-Ser(175)), beta-MSH (Asp(159)-Ser(175)), and beta-endorphin (Tyr(178)-Gln(208)). No region homologous to gamma-MSH/joining peptide (a tetrapod POMC feature) was found. Amino acid sequence identity was high with other teleostean species considered (tilapia: 73%) and lower with elasmobranchs (dogfish: 42%). However, the presumed biologically active peptides were highly conserved within all species considered: alpha-MSH (93-100%), ACTH (80-95%) and beta-endorphin (54-90%). Real-time PCR allowed us to quantity the expression of the POMC in different tissues of the sea bass: pituitary gland, liver, gonad and head kidney. No significant PCMC expression was found in the integument. In pituitary gland, gonads, head kidney and liver, POMC expression was respectively, 1.26 X 10(10), 2.67 X 10(5), 2.06 X 10(4) and 1.67 X 10(4) copies/mug mRNA.
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