期刊
FERTILITY AND STERILITY
卷 79, 期 -, 页码 770-778出版社
ELSEVIER SCIENCE INC
DOI: 10.1016/S0015-0282(02)04834-3
关键词
endometriosis; peritoneum; mesothelium; confocal; laser-scanning microscopy
Objective: To evaluate endometrial adhesion and invasion of peritoneal mesothelium. Design: Descriptive study using confocal laser-scanning microscopy. Setting: University-based laboratory. Patient(s): Women undergoing surgery for benign conditions. Intervention(s): Fluorescence-labeled peritoneal mesothelial cells (PMCs) were grown on coverslips. Fluorescence-labeled endometrial stromal cells (ESCs) and epithelial cells (EECs) and myometrial cells (Myos) were plated on the PMCs. Cultures were examined at 1, 6, 12, and 24-27 hours with differential interference contrast and confocal laser-scanning microscopy. Main Outcome Measure(s): Demonstration of adherence and invasion of endometrial cells through peritoneal mesothelium. Result(s): At 1 hour, there was adherence of the ESCs, EECs, and Myos on the perimeter of PMCs. There was no invasion by the Myos. By 6 hours, ESCs and EECs spread over the surface of the PMCs and extended cell processes through PMC junctions. Extension of pseudopodia under the PMCs followed. By 12 hours, there was vacuolization and lifting of PMCs that had been undermined by endometrial cells. Conclusion(s): This is the first time-phase study to demonstrate adherence and the process of invasion of endometrial cells through the mesothelium. The application of three-dimensional confocal laser-scanning microscopy is a novel technique that can be used to further examine mechanisms involved in the pathogenesis of the early endometriotic lesion. (Fertil Steril((R)) 2003;79(Suppl 1):770-8. (C) 2003 by American Society for Reproductive Medicine.).
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据