期刊
DEVELOPMENTAL AND COMPARATIVE IMMUNOLOGY
卷 27, 期 3, 页码 189-196出版社
PERGAMON-ELSEVIER SCIENCE LTD
DOI: 10.1016/S0145-305X(02)00099-X
关键词
immulectin; pattern recognition; C-type lectin; carbohydrate recognition domains lipopolysaccharide; phenoloxidase activation; insect immunity; tobacco hornworm
资金
- NIGMS NIH HHS [GM66356, R37 GM041247, GM41247] Funding Source: Medline
We previously reported the isolation of a lipopolysaccharide (LPS)-specific immulectin-2 from the tobacco hornworm, Manduca sexta [J. Biol. Chem. 275 (2000) 37373]. Immulectin-2 is a C-type lectin that is present at a constitutively low level in hemolymph of naive larvae, and its synthesis is induced after injection of Gram-negative bacteria or LPS. Immulectin-2 contains two carbohydrate recognition domains. It binds to LPS and stimulates prophenoloxidase activation in plasma. In this paper, we focus on properties of carbohydrate recognition domain-2 of immulectin-2 and the biological functions of immulectin-2 in immune responses. The carboxyl-terminal carbohydrate recognition domain (CRD2) of immulectin-2 was able to bind bacterial LPS. Binding of recombinant CRD2 to LPS stimulated activation of prophenoloxidase in plasma. Injection of antiserum against immulectin-2 into M. sexta larvae inhibited clearance of a Gram-negative bacterial pathogen, Serratia marcescens, and decreased survival of infection. These results suggest that immulectin-2 plays an important role in the immune system of M. sexta, and helps to protect the animal from Gram-negative bacterial infections. (C) 2002 Elsevier Science Ltd. All rights reserved.
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