Optimizing multiplex Polymerase Chain Reaction method for specific, sensitive and rapid detection of Salmonella sp., Escherichia coli and Bifidobacterium sp in chick gastrointestinal tract

In the present study, a multiplex Polymerase Chain Reaction (mPCR)-based assay was developed for the simultaneous identification of Salmonella sp., Escherichia coli and Bifidobacterium sp., that isolated from broiler gastrointestinal in Iran. In order to detect these bacteria species, we were used a set of specific primers that produce different fragment size. Our results showed that all tested gastrointestinal segments were contaminated by these species and therefore the mPCR method is a appropriate method to identify these species directly from poultry gastrointestinal samples. There was no difference in the sensitivity of this method between samples of different gastrointestinal segments. This method has the potential to save considerable time and effort within the laboratory. Our developed mPCR is fast, sensitive, specific and can be used as a reliable choice for routine detection method of these bacteria species in chick gastrointestinal contents.