期刊
FEBS LETTERS
卷 538, 期 1-3, 页码 60-64出版社
ELSEVIER SCIENCE BV
DOI: 10.1016/S0014-5793(03)00133-9
关键词
NPP; nucleotide pyrophosphatase/phosphodiesterase; lysophospholipase D; autotaxin; cell motility; catalytic mechanism; PC-1
Autotaxin (NPP2) is a tumor cell motility-stimulating factor that displays both a nucleotide pyrophosphatase/phosphodiesterase activity and a recently described lysophospholipase D activity. The hydrolysis of nucleotides is a metal-assisted reaction that occurs via a nucleotidylated threonine in the catalytic site. We show here that the catalytic site threonine and the metal-coordinating residues are also essential for the hydrolysis of lysophospholipids. In comparing the substrate specificity of NPP2 and the closely related NPP1 and NPP3, we found that only NPP2 displayed a lysophospholipase D activity, whereas NPP1 and NPP3 had a much higher nucleotide pyrophosphatase activity. (C) 2003 Published by Elsevier Science B.V. on behalf of the Federation of European Biochemical Societies.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据