期刊
FEBS LETTERS
卷 538, 期 1-3, 页码 159-162出版社
ELSEVIER SCIENCE BV
DOI: 10.1016/S0014-5793(03)00172-8
关键词
P2X(7); channel function; cytolytic pore; antibody
P2X(7) receptor/channels mediate ATP-induced apoptosis in a range of cells including lymphocytes. HEK293 cells were transfected with wild-type human P2X7 receptor or site-directed mutant constructs (K193A, K311A and E496A) known to be non-functional from measurements of barium/ethidium influx in the presence of ATP or 2',3'-O-(4-benzoylbenzoyl)-ATP. An antibody was designed against an epitope from a loop adjacent to the extracellular ATP site. The epitope was unavailable in cells expressing normal functional surface receptors. Non-functional surface receptors as well as intracellular receptors selectively bound the antibody. So did B-lymphocytes from chronic lymphocytic leukemia patients expressing non-functional (E496A) mutant receptor. (C) 2003 Published by Elsevier Science B.V. on behalf of the Federation of European Biochemical Societies.
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