期刊
ASIAN JOURNAL OF ANDROLOGY
卷 13, 期 3, 页码 432-437出版社
SHANGHAI INST MATERIA MEDICA
DOI: 10.1038/aja.2010.159
关键词
Leydig cells; oxidative damage; reactive oxygen species; testosterone
资金
- Shin Kong Wu Ho-Su Memorial Hospital [SKH-TMU-93-43]
Testosterone replacement therapy has benefits for aging men and those with hypogonadism. However, the effects of exogenous testosterone on Leydig cells are still unclear and need to be clarified. In this report, we demonstrate that testosterone supplementation can reduce oxidative damage in Leydig cells. The TM3 Leydig cell line was used as an in vitro cell model in this study. Cytoprotective effects were identified with 100-nmol l(-1) testosterone treatment, but cytotoxic effects were found with >= 500-nmol l(-1) testosterone supplementation. Significantly reduced reactive oxygen species (ROS) generation, lipid peroxide contents and hypoxia induction factor (HIF)-1 alpha stabilization and activation were found with 100-nmol l(-1) testosterone treatment. There was a 1.72-fold increase in ROS generation in the 500-nmol l(-1) compared to the 100-nmol l(-1) testosterone treatment. A 1.58-fold increase in steroidogenic acute regulatory protein (StAR) expression was found in 50-nmol l(-1) testosterone-treated cells (P < 0.01). Chemically induced hypoxia was attenuated by testosterone supplementation. Leydig cells treated with low-dose testosterone supplementation showed cytoprotection by decreasing ROS and lipid peroxides, increasing StAR expression and relieving hypoxia stress as demonstrated by HIF-1 alpha stabilization. Increased oxidative damage was found with >= 500-nmol l(-1) testosterone manipulation. The mechanism governing the differential dose effects of testosterone on Leydig cells needs further investigation in order to shed light on testosterone replacement therapy. Asian Journal of Andrology (2011) 13, 432-437; doi:10.1038/aja.2010.159; published online 7 February 2011
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据