期刊
CANCER LETTERS
卷 192, 期 1, 页码 59-65出版社
ELSEVIER SCI IRELAND LTD
DOI: 10.1016/S0304-3835(02)00682-1
关键词
leukemic cells; normal lymphocytes; lectins; lectin-affinity chromatography
类别
Utilization of leukemic T-cells from normal ones, using lectin-affinity adsorbents, is described. CNBr-activated Sepharose 6MB was covalently coupled to Soybean (SBA) or Dolichos Biflorus Agglutinins (DBA), then serves as an affinity probe for separation of leukemic T-cells from normal lymphocytes. The normal lymphocytes were removed almost completely by phosphate buffered saline (Ca2+ and Mg2+ free) (PBS(-)) from lectin-affinity column. More than 80% of the leukemic T-cells were retained on the lectin-affinity adsorbent, whereas another 10-15% were easily removed by PBS(-). There was a very good linear correlation between percent of cells, retained on the lectin-affinity adsorbent and percent of cells, interacting with the respective free lectin (r = 0.97 for SBA, and r = 0.93 for DBA). The viability of normal lymphocytes was not influenced after passing through the columns. In the case of leukemic T-cells - about 90% of the easily removed cells were dead, and another 10% were viable cells, non-interacting with DBA or SBA. (C) 2002 Elsevier Science Ireland Ltd. All rights reserved.
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