期刊
JOURNAL OF CHROMATOGRAPHY A
卷 990, 期 1-2, 页码 259-269出版社
ELSEVIER SCIENCE BV
DOI: 10.1016/S0021-9673(03)00050-5
关键词
optimisation; fluoroquinolones; quinolones; antibiotics
The applicability of a capillary zone electrophoresis-electrospray ionisation tandem mass spectrometric (wCZE-ESI-MS-MS) method for the separation of nine fluoroquinolones was investigated. Method optimisation involved systematic trouble-shooting starting with the type and duration of capillary pre-washing and conditioning, the choice of both the CE run buffer, MS sheath liquid, CE run potential, ESI spray voltage, sheath gas flow-rate, MS capillary voltage and CE capillary and MS capillary temperatures. Another extremely important factor was found to be the degree to which the CE capillary protrudes into the ESI chamber as well as whether or not sheath gas and spray voltage are employed during the CE injection or not. The importance of the latter has, to our knowledge, not been addressed elsewhere. Nine fluoroquinolones have been separated and detected in a single run by this technique. (C) 2003 Elsevier Science B.V. All rights reserved.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据