期刊
ANALYTICA CHIMICA ACTA
卷 481, 期 1, 页码 119-126出版社
ELSEVIER
DOI: 10.1016/S0003-2670(03)00070-9
关键词
apple; chlorophyll; fruit quality; non-destructive; visible spectrum
Non-destructive, rapid, instrumental tools in fruit production are required for predicting the optimum harvest window and monitoring fruit quality during shelf life. The degree of chlorophyll degradation is a sensitive indicator for fruit maturation and ageing. Adequate indices for chlorophyll prediction by means of non-destructive spectral analysis have been studied in the fields of photosynthesis research and remote sensing developments. However, an evaluation of these indices and multivariate linear regression models does not exist so far for predicting the fruit chlorophyll content. Spectral transmittance recordings in the visible wavelength range were carried out on apple fruit Malus domestica Borkh. 'Elstar' (n = 99) and 'Jonagold' (n = 117). The fruit chlorophyll a content of 'Elstar' apples was measured wet-chemically and predicted by means of the specific indices: NDVI, Tr-698/Tr-760, red-edge, TrII on Tr'(lambda), TrII, and RVSI with the correlation coefficients of determination R-2 = 0.84, 0.81, 0.75, 0.81, 0.15, and 0.67, respectively. Partial least-squares (PLS) calibration models were built using calibrated spectra (630-730 nm), first derivative of spectra and second derivative of spectra yielding multivariate correlation coefficients of determination R-2 = 0.81, 0.86, and 0.92, respectively. Similar results were found for 'Jonagold' apple fruit. Linear regression of indices and PLS calibration models were empirically tested on the chlorophyll a content of apple fruit measuring the same cultivars grown in a different seasons and growing locations. In the case of 'Elstar' apple fruit, correlation coefficients of fruit chlorophyll a content analysed wet-chemically as well as fruit maturation measured as calendar weeks were similar to those in the calibration experiment. Application of linear regression equations of indices and PLS models on spectra of 'Jonagold' apple fruit led to less accurate results for those methods, which use wavelengths above 720 nm as indicative range. (C) 2003 Elsevier Science B.V. All rights reserved.
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