期刊
COLLOIDS AND SURFACES A-PHYSICOCHEMICAL AND ENGINEERING ASPECTS
卷 215, 期 1-3, 页码 25-32出版社
ELSEVIER
DOI: 10.1016/S0927-7757(02)00416-8
关键词
folded drop surface; protein adsorption; liquid/liquid interface; axisymmetric drop shape analysis
The adsorption behaviors of three proteins, human serum albumin, beta-lactoglobulin and beta-casein, respectively, at the chloroform/water interface were studied by using the pendent drop technique. A drop was produced by immersing the pure chloroform solvent into protein buffer solution through a quartz capillary. It has been found that a folded drop surface can be formed by the adsorption of each protein after certain time. The 'skin-like' film was assumed to be composed of adsorption layer of protein. By using a 50 mum capillary, we have obtained a hundreds of micrometer droplet. The adsorption kinetics of each protein was recorded by using the commercial software of axisymmetric drop shape analysis to understand the forming process of the 'skin-like' film. (C) 2002 Elsevier Science B.V. All rights reserved.
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